Abstract: Laser spectroscopy, which are spectroscopy techniques using laser as a light source, have attracted widespread attention from scientists over the word due to their good monochromaticity, high brightness, strong directionality and coherence. Compared with traditional spectroscopic techniques, they have higher sensitivity, resolution and broader application prospects. Nowadays, the rapid development of micro-nano optics and intelligent algorithms provides new opportunities to broaden the application fields of laser spectroscopy. The special issue on laser spectroscopy in 2025 has published 12 papers, including 3 review articles and 9 original articles. This special issue aims to showcase the main research progress and achievements in laser spectroscopy over recent years, allowing readers to have a deeper understanding of the current status, trends, and application prospects of laser spectroscopy research, and also to serve as a reference for the scientific reseraches and implementation.