Abstract: In order to adjust the position of the secondary mirror of Cassegrain telescope with large field of view, a computer aided adjustment method of two-step sensitivity matrix model was proposed. Based on the analysis of the shortcomings of the traditional sensitivity matrix method of the two order model, a fine tuning step was added based on the characteristics of the sensitivity matrix and the traditional sensitivity matrix method was improved. For the Cassegrain system, the relationship between the Zernike coefficients and the misalignment was analyzed, and the calibration simulation of Cassegrain system with 300 mm aperture and 0.6° field of view was carried out. The simulation results show that after correction by traditional sensitivity matrix method, the mean values of offset along x, y, z axes and tilt around x, y axes are -0.0684 mm, -0.0892 mm, 0.0015 mm, 0.0498° and -0.0444°, respectively, and the full field wavefront aberration RMS is less than 0.1λ(λ=632.8 nm). After correction by two step sensitivity matrix correction method, the mean values are -0.0018 mm, -0.0012 mm, 0.0002 mm, 0.0008° and -0.0012°, respectively, and the full field wavefront aberration RMS is less than 0.03λ, which is obviously superior to the traditional sensitivity matrix method.