Abstract: A regionally controllable super hydrophobic/super hydrophilic mixed surface was prepared by laser ablation, and the effects of pre-wetting on the surface wettability of samples under water and oil were studied, as well as the stability of the surface wettability of samples. The results show that pre-wetting can change the oil contact angle underwater and water contact angle under-oil on the sample surface, and also change the behavior of bubbles on the surface. After the samples were soaked in water, heated or exposed to air, the super hydrophilic surface showed wettability transformation while the super hydrophobic surface was relatively stable. The sample can maintain long-term stability sealed dry preservation at room temperature. The results are of great significance for oil-water separation, oil-gas separation and bubble control in aqueous media.