Customized anterior segment photoacoustic imaging for ophthalmic burn evaluation <em>in vivo</em>

Huangxuan Zhao; Ke Li; Fan Yang; Wenhui Zhou; Ningbo Chen; Liang Song; Chuansheng Zheng; Zhicheng Liu; Chengbo Liu

doi:10.29026/oea.2021.200017

Article navigation > Opto-Electronic Advances > 2021 Vol. 4 > No. 6 > 200017

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Zhao HX, Li K, Yang F, Zhou WH, Chen NB et al. Customized anterior segment photoacoustic imaging for ophthalmic burn evaluation in vivo. Opto-Electron Adv 4, 200017 (2021). doi: 10.29026/oea.2021.200017

Citation:

Zhao HX, Li K, Yang F, Zhou WH, Chen NB et al. Customized anterior segment photoacoustic imaging for ophthalmic burn evaluation in vivo. Opto-Electron Adv 4, 200017 (2021) . doi: 10.29026/oea.2021.200017

Original Article Open Access

Customized anterior segment photoacoustic imaging for ophthalmic burn evaluation in vivo

1.
Department of Radiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China
2.
Research Laboratory for Biomedical Optics and Molecular Imaging, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, Shenzhen 518055, China
3.
School of Biomedical Engineering, Capital Medical University, Beijing 100069, China
4.
Beijing Key Laboratory of Fundamental Research on Biomechanics in Clinical Application, Beijing 100069, China

More Information

^†These authors contributed equally to this work
Corresponding authors: CS Zheng, E-mail: hqzcsxh@sina.com; ZC Liu, E-mail: zcliu@ccmu.edu.cn; CB Liu, E-mail: cb.liu@siat.ac.cn

Received Date 19 June 2020

Accepted Date 21 July 2020

Available Online 20 June 2021

Published Date 20 June 2021

Abstract

Abstract

Photoacoustic imaging has many advantages in ophthalmic application including high-resolution, requirement of no exogenous contrast agent, and noninvasive acquisition of both morphologic and functional information. However, due to the limited depth of focus of the imaging method and large curvature of the eye, it remains a challenge to obtain high quality vascular image of entire anterior segment. Here, we proposed a new method to achieve high quality imaging of anterior segment. The new method applied a curvature imaging strategy based on only one time scanning, and hence is time efficient and more suitable for ophthalmic imaging compared to previously reported methods using similar strategy. A custom-built photoacoustic imaging system was adapted for ophthalmic application and a customized image processing method was developed to quantitatively analyze both morphologic and functional information in vasculature of the anterior segment. The results showed that the new method improved the image quality of anterior segment significantly compared to that of conventional high resolution photoacoustic imaging. More importantly, we applied the new method to study ophthalmic disease in an in vivo mouse model for the first time. The results verified the suitability and advantages of the new method for imaging the entire anterior segment and the numerous potentials of applying it in ophthalmic imaging in future.
- photoacoustic imaging /
- ophthalmic imaging /
- morphologic and functional information /
- ophthalmic disease /
- anterior segment

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References

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